abstract：:A virus (PBCV) from the symbiotic Chlorella-like algae of Paramecium bursaria has been characterized by sedimentation field flow fractionation (SdFFF). The method yields effective molecular weight M' (molecular weight X buoyancy factor) with a precision of better than 5% under a variety of experimental conditions. The...

journal_title：Journal of virological methods

authors： Yonker CR,Caldwell KD,Giddings JC,Van Etten JL

更新日期：1985-06-01 00:00:00

abstract：:A simple immunoperoxidase assay (IPA), adapted for detection of serum IgM antibodies to cytomegalovirus (CMV) is described. The antigen consisted of CMV infected human embryonic fibroblasts or isolated nuclei. The sera were absorbed with aggregated gamma-globulins prior to testing. Rabbit anti-human IgM peroxidase con...

journal_title：Journal of virological methods

authors： Singer Y,Kimmel N,Sarov I

更新日期：1985-05-01 00:00:00

abstract：:The use of 0.1% aqueous solution of polylysine (poly-L-lysine) is proposed as a prior step to negative staining of viral or particle suspensions. Particles spread better on films precoated with polylysine than with other substances used for the same purpose. This applies particularly to samples from sucrose or CsCl gr...

journal_title：Journal of virological methods

authors： Barth OM

更新日期：1985-05-01 00:00:00

abstract：:The subclass distribution of antiviral antibodies to three herpes viruses was studied in a population of healthy blood donors. Subclassification by monoclonal antibodies led to the identification of certain viral IgG patterns. IgG1 appeared to be formed in response to almost all CMV, HSV-1 and VZV infections. A higher...

journal_title：Journal of virological methods

authors： Gilljam G,Sundqvist VA,Linde A,Pihlstedt P,Eklund AE,Wahren B

更新日期：1985-03-01 00:00:00

abstract：:A method is described for detecting enteroviruses in both freshwater and marine sediments. Viruses were recovered from sediments by elution into 6% beef extract at pH 9.0 and concentration with polyethylene glycol 6000. The recovery efficiency ranged from 6 to 55% for marine sediments and 16 to 77% for freshwater sedi...

journal_title：Journal of virological methods

authors： Lewis GD,Loutit MW,Austin FJ

更新日期：1985-02-01 00:00:00

abstract：:Tissue culture ID50 and plaque assays for the Autographa californica nuclear polyhedrosis virus, using the Spodoptera littoralis cell line HPB-SL, were developed. Direct comparison using these assay methods showed that these cells were as susceptible to infection as the more commonly used Spodoptera frugiperda cell li...

journal_title：Journal of virological methods

authors： Roberts PL

更新日期：1985-01-01 00:00:00

abstract：:We show that the immuno-gold technique adapted to electron microscopy is a sensitive method for in situ localization of viral proteins in plant cells. Using antisera raised against cauliflower mosaic virus and the viroplasm major protein (VmP) we obtained a selective labelling of the viral particles and of the viropla...

journal_title：Journal of virological methods

authors： Giband M,Stoeckel ME,Lebeurier G

更新日期：1984-12-01 00:00:00

abstract：:Details of a procedure for detecting double-stranded RNA (dsRNA) in virus and viroid infected tissue extracts using serologically specific electron microscopy are given. A method for staining dsRNA, based on in situ formation of uranyl phosphate, that consistently permits the examination of dsRNA by electron microscop...

journal_title：Journal of virological methods

authors： Derrick KS,French RC,Clark CA,Gabriel CJ

更新日期：1984-12-01 00:00:00

abstract：:A simple method for evaluating the virucidal activity of water-soluble antiseptic and disinfectant products using poliovirus type 1 (Sabin strain) is described. Using a commercial concentrator, kinetic studies of four products were carried out. It was shown that 2% glutaraldehyde and 5% povidone iodine are rapidly vir...

journal_title：Journal of virological methods

authors： Boudouma M,Enjalbert L,Didier J

更新日期：1984-12-01 00:00:00

abstract：:A method is described that allows the rapid screening of field isolates of rotavirus for the detection of specific viral RNA segments. Cloned cDNA copies of viral genomic RNAs are employed for detection in the assay which makes use of the dot-hybridization technique of Thomas (1980). The assay developed was shown to b...

journal_title：Journal of virological methods

authors： Pedley S,McCrae MA

更新日期：1984-10-01 00:00:00

abstract：:Paired sera from 784 patients with symptoms of acute respiratory disease were examined for antibodies against influenza A, B and parainfluenza (1 and 3) viruses by complement fixation (CF) and enzyme immunoassay (EIA). The internal variation of the EIA test results was low and an increase of 0.250 in absorbance values...

journal_title：Journal of virological methods

authors： Julkunen I,Kleemola M,Hovi T

更新日期：1984-08-01 00:00:00

abstract：:Hepatitis B viral (HBV) DNA was detected in a hepatoma cell line which produces hepatitis B surface antigen (HBsAg) and in patients with acute hepatitis B. The serum of one patient with acute hepatitis B was found to be infectious when injected i.v. into a chimpanzee up to a dilution of 10(-8). Hepatitis B surface ant...

journal_title：Journal of virological methods

authors： Feinman SV,Berris B,Guha A,Sooknanan R,Bradley DW,Bond WW,Maynard JE

更新日期：1984-05-01 00:00:00

abstract：:The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show ...

journal_title：Journal of virological methods

authors： Ellis DS,Smith MD,Lloyd G,Bowen ET,Simpson DI

更新日期：1984-02-01 00:00:00

abstract：:As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...

journal_title：Journal of virological methods

authors： Neurath AR,Strick N,Miller K,Waldman AA

更新日期：1984-02-01 00:00:00

abstract：:A sensitive enzyme-linked immunosorbent assay (ELISA) has been developed which can detect serotype and group-specific antibodies (IgG) to fowl adenovirus serotypes 2, 3 and 4. The chickens produced principally type-specific antibodies after a single oral inoculation of virus which enabled that strain to be identified ...

journal_title：Journal of virological methods

authors： Mockett AP,Cook JK

更新日期：1983-12-01 00:00:00

abstract：:The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

journal_title：Journal of virological methods

authors： Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

更新日期：1983-09-01 00:00:00

abstract：:A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable fo...

journal_title：Journal of virological methods

authors： Ikram H,Prince AM

更新日期：1983-09-01 00:00:00

abstract：:A rapid and sensitive assay was developed to analyse the sensitivity of wild type HSV-1 and HSV-2 isolates with respect to a battery of antiviral substances. In the viral sensitivity assay, human embryonic lung fibroblasts are incubated with the virus isolate and different concentrations of the antivirals. After 1-3 d...

journal_title：Journal of virological methods

authors： Wahren B,Harmenberg J,Sundqvist VA,Levén B,Sköldenberg B

更新日期：1983-03-01 00:00:00

abstract：:Several Togaviridae of the alphavirus and flavivirus genera agglutinate trypsinized human group O erythrocytes (THOE) (Shortridge and Hu, 1976). Haemagglutinin titers of Semliki Forest virus (SFV) and Japanese encephalitis virus (JEV) measured with THOE were equivalent to, if not higher than, those obtained with Embde...

journal_title：Journal of virological methods

authors： Sedmak JJ,Dixon M,Schoenherr C,Sabran JL,Grossberg SE

更新日期：1983-02-01 00:00:00

abstract：:The recently developed semi-automatic Hepatube system was evaluated in comparison to another radioimmunoassay for the detection of hepatitis B surface antigen (HBsAg), the manual Ausria II-125 test. After incubation of serum in anti-HBs coated tubes, the Hepatube system uses a machine to wash the tubes and to add trac...

journal_title：Journal of virological methods

authors： de Vries J,Kruining J,Heijtink RA

更新日期：1983-02-01 00:00:00

abstract：:A direct ELISA was developed for determination of IgM antibody to varicella zoster virus (VZV). With this sensitive method VZV IgM antibodies were detected in all patients with a varicella and in 84% with a herpes zoster infection. All but one of 28 renal allograft recipients had previously had varicella. A primary in...

journal_title：Journal of virological methods

authors： Sundqvist VA

更新日期：1982-11-01 00:00:00

abstract：:A simple haemadsorption procedure for the detection of isotype specific antibodies to R.S. virus has been developed. Correlation between detection of R.S. virus IgG antibodies by mixed reverse (solid phase) passive antiglobulin haemadsorption (MRSPAH) and other serological procedures has been obtained. IgA antibody wa...

journal_title：Journal of virological methods

authors： Cranage MP,Coombs RR

更新日期：1982-11-01 00:00:00

abstract：:The applicability of the enzyme immunoassay technique to the detection of group and interspecies determinants of C-type retroviral proteins was tested. For this purpose four groups of C-type retroviruses (MuLV, FeLV, SiSV/GaLV, and BaEV/RD114) were assayed for group-specific and interspecies reactivities of their p30 ...

journal_title：Journal of virological methods

authors： Schetters H,Hehlmann R,Erfle V,Saxinger C

更新日期：1982-11-01 00:00:00

abstract：:Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy, when diluted in sta...

journal_title：Journal of virological methods

authors： Hovi T,Väisänen V,Ukkonen P,von Bonsdorff CH

更新日期：1982-09-01 00:00:00

abstract：:IgM antibody capture radioimmunoassay (MACRIA) and enzyme immunoassay (MACEIA) were compared with immunofluorescence (IF) for detecting specific IgM antibody in 99 sera from 76 infants with confirmed congenital rubella, and 61 sera from a comparative group of 59 infants who had miscellaneous abnormalities but in whom ...

journal_title：Journal of virological methods

authors： Chantler S,Evans CJ,Mortimer PP,Cradock-Watson JE,Ridehalgh MK

更新日期：1982-08-01 00:00:00

abstract：:Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the ...

journal_title：Journal of virological methods

authors： Beards GM

更新日期：1982-08-01 00:00:00

abstract：:This report describes the conditions for the use of aluminum chloride (AlCl3) in growth and maintenance media for the suppression or inhibition of simian foamyviruses (SFV) in primary baboon kidney (BAK) and rabbit kidney (RK) cell cultures. When RK cells were planted in medium containing AlCl3, infected with SFV, and...

journal_title：Journal of virological methods

authors： Weiss SA,Heberling RL,Kalter SS

更新日期：1982-05-01 00:00:00

abstract：:Cell membranes were prepared from herpes simplex virus (HSV) type 1-infected cells and solubilized with a low concentration of sodium deoxycholate. The supernatant after ultracentrifugation was used as antigen in a newly developed solid phase assay, diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA). Antig...

journal_title：Journal of virological methods

authors： Jeansson S,Elwing H,Nygren H,Olofsson S

更新日期：1982-04-01 00:00:00

abstract：:Purified hepatitis B surface antigen (HBsAg) of subtype ay was solubilized in guanidinium chloride and submitted to chromatography on Sepharose 4B in the presence of guanidinium chloride. The polypeptides P1 (Mr = 24,000) and P2 (Mr = 29,000) were eluted in the same fraction with a minor contaminant (Mr = 40,000). Lar...

journal_title：Journal of virological methods

authors： Hubert M,Barin F,Goudeau A

更新日期：1982-01-01 00:00:00

abstract：:Rubella virosomes were prepared from performed liposomes and detergent solubilized viral hemagglutinin. The liposomes were made from lecithin/dicetyl phosphate (3.5 : 1) films resuspended in NTE buffer and sonicated. Viral hemagglutinin was prepared from purified virus after solubilization with Triton X-100 and centri...

journal_title：Journal of virological methods

authors： Trudel M,Marchessault F,Payment P

更新日期：1981-11-01 00:00:00

abstract：:A detailed growth and purification scheme suitable for producing relatively large quantities of fully active, pure SV40 is presented together with data on recovery and purity at each step of the procedure. The scheme was designed to prevent the initial binding of virus to cell components as well as contamination of th...

journal_title：Journal of virological methods

authors： Rosenberg BH,Deutsch JF,Ungers GE

更新日期：1981-10-01 00:00:00

abstract：:A solid-phase enzyme-linked immunoassay using a fluorogenic substrate (4-methylumbelliferyl-beta-D-galactopyranoside) was developed. Antibodies were covalently linked to glutaraldehyde-activated 96-well aminopolystyrene plates. Antigens from test samples were adsorbed to the solid phase and detected using antibodies c...

journal_title：Journal of virological methods

authors： Neurath AR,Strick N

更新日期：1981-10-01 00:00:00

abstract：:Recently, the seroneutralization, hemagglutination inhibition, and the ELISA procedures were used to determine the presence of serum antibody to the pneumonia virus of mice in rat sera. This study was initiated to evaluate the reliability of these procedures to detect specific antibodies in our laboratory animal speci...

journal_title：Journal of virological methods

authors： Descôteaux JP,Payment P

更新日期：1981-09-01 00:00:00

abstract：:Immune electron microscopy (IEM) cannot be used successfully for structures that do not have recognisable morphology. However, at least some of these structures or components are related antigenically to recognisable antigens or viruses. We have therefore mixed unknown antigens with known markers and looked for the pr...

journal_title：Journal of virological methods

authors： Almeida JD,Skelly J,Howard CR,Zuckerman AJ

更新日期：1981-02-01 00:00:00

abstract：:Two modifications of Hepanostika were studied in order to improve this enzyme-immunoassay with regard to duration of the test, sensitivity and reading of test results. Test conditions and composition of some components of the test system were modified. With the first modification the test could be performed within 3 h...

journal_title：Journal of virological methods

authors： Wolters G,Kuijpers LP,Croese J

更新日期：1980-12-01 00:00:00

abstract：:High-titered phage stocks are required for carrying out genetic and physicochemical studies on bacteriophages. This study describes a simple method for the concentration of coliphages MS-2 and T2 by charge-modified filters. Phage lysates were first clarified by filtration through serum-coated membrane filters. The cla...

journal_title：Journal of virological methods

authors： Goyal SM,Zerda KS,Gerba CP

更新日期：1980-01-01 00:00:00

abstract：:Lymphoblastoid B.95-8 cells were cultured for four months and three weeks in the presence of increasing concentrations (50--200 microgram/ml) of phosphonoacetic acid (PAA). Several weeks after removal of the PAA, the cultures, in parallel with untreated B.95-8 cells, were tested for the presence of: 1) Epstein-Barr vi...

journal_title：Journal of virological methods

authors： Margalith M,Manor D,Goldblum N

更新日期：1980-01-01 00:00:00

abstract：:Formalinized chick erythrocytes agglutinated with Japanese encephalitis virus antigens over a wider range of pH (6.0-7.2) than fresh erythrocytes (pH 6.4-6.8). The virus antigens were unstable below pH 7.0 and therefore the use of the formalinized erythrocytes made it possible for a one-diluent system of the hemagglut...

journal_title：Journal of virological methods

authors： Ito H,Iwasa S

更新日期：1980-01-01 00:00:00